Microscopy and spectroscopy
For decades, optical microscopy has played an important role in the observation of cell biology. In microscopy, as for all imaging optical system, the spatial resolution is limited. Recently, several new approaches have been developed to overcome this limit, particularly in fluorescence microscopy. We investigate the use of saturation of the two-photon absorption fluorescence excitation for improving the spatial resolution. We are also investigating the use of quantum optics technologies for high and low frequency Raman spectroscopy.
Picture: Plasmonic Raman sensor with free space resonant excitation and waveguide harvesting of Raman signal.
Picture: 3D imaging of fluorescent microspheres embedded in HeLa cells by multiphoton microscopy with resolution enhancement by fluorescence saturation